Part:BBa_K4591011

T500-Para-lox66-PdegQ-lox71-MazF-T500

Usage and Biology

Our system was to be able to work perfectly in the environment, but we have to consider that the trigger conditions of the suicide switch are not suitable for the stage of cultivation and modification of engineered bacteria, since the concentrations of engineered bacteria need to be high at that stage. Therefore, it is imperative to investigate novel methods for regulating the suicide switch.

Cre-Loxp recombination is a site-specific recombinase technology, which was used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. The effectiveness of this technology hinges on the presence of flanking sequences, known as Recombinase Target Sites (RTSs), that mark recombination sites.If the RTSs are arranged in head-to-head orientation, the sequence within the RTSs is flipped (called inversion).

With this Flip Module, we can precisely control the on and off of the Suicide Switch by ingenious genetic circuit design and inducing the expression of cre recombinase.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 324
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 155
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 137